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Dr. J. Manuel Perez

J. Manuel Perez, Ph.D.
Associate Professor - Joint Appointment in NanoScience Technology Center and Chemistry with a Secondary Joint in Biomedical Sciences
Phone: 407-882-2843
E-mail: jmperez@ucf.edu
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Education

  • Postdoctoral Fellowship — MGH-Harvard Medical School, Boston
  • Ph.D. — Boston University, Boston MA
  • M.S/B.S. Chemistry — University of Puerto Rico, Mayaguez, PR

Prior Positions

  • Instructor in Chemistry, Center for Molecular Imaging Research, MGH-Harvard Medical School, 2003-2005

Affiliations

  • Department of Chemistry
  • Biomolecular Science Center

Research

Research in my laboratory focuses on the development of nanoparticle technologies and their application in nanomedicine, molecular imaging, and molecular diagnostics. My primary interest is the design of target-specific nanoparticle-based conjugates with potential imaging and therapeutic applications in cancer and bacterial infections. Other areas of current interest are the:

  1. Development of nanoparticle technologies and biosensors to detect molecular markers associated with disease in point-of-care diagnostic applications and for the detection of harmful pathogens and toxins
  2. Development of novel multifunctional nanoparticles for the imaging and treatment of cancer
  3. Development of sensitive and high throughput pathogen and toxin detection using magnetic nanoparticles and NMR/MRI detection
  4. Design of targeted polymer coated cerium oxide nanoparticles as antioxidant nanoagents and nanosensors

Current Funding

  • NIH/NIGMS R01: An integrated NMR/magnetic nanosensor system for detection of bacteria and toxins
    Funding Period: 9/2008-8/2013

Previous Funding

  • NIH/NCI K01-Career Award: Development of magnetic sensors for MRI cancer research
    Funding Period: 8/2003-7/2008

Select Publications

2012

Santra, S., Jativa, S.D., Kaittanis C., Santiesteban, Normand, G. Grimm, J., Perez, J.M. Gadolinium-Encapsulating Iron Oxide Nanoprobe as Activatable NMR/MRI Contrast Agent, ACS Nano 2012, 8, 7281-7294

Asati, A., Lehmkuhl, D., Diaz, D., Perez, J.M. Nanoceria Facilitates the Synthesis of Poly(o-phenylenediamine) with pH-Tunable Morphology, Conductivity, and Photoluminescent Properties. Langmuir 2012,28, 13066-13071

Boohaker, R.J., Zhang, G., Lee, M.W., Nemec, K.N., Santra, S., Perez, J.M., Khaled, A.R. Rational development of a cytotoxic peptide to trigger cell death, Mol. Pharmaceutics 2012, 9, 2080-2093

Kaittanis, C., Boukhriss, H., Santra S., Naser, S. A., Perez, J.M. Rapid and sensitive detection of an intracellularpathogen in human peripheral leukocytes with hybridizing magnetic relaxation nanosensors, PLOS One, 2012, 7(4): e35326. doi:10.1371/journal.pone.0035326

Santiesteban, O.J., Kaittanis, C, Santra S., Perez, J.M. Assessment of molecular interactions through magnetic relaxation, Angew. Chem., Int. Ed. 2012, 51, 6728-6732

Kaittanis, C, Santra S., Asati, A., Perez, J.M. A cerium oxide nanoparticle-based device for the detection of chronic inflammation via optical and magnetic resonance imaging, Nanoscale 2012, 4, 2117

2011

Santra, S., Kaittanis C., Santiesteban, O.J., Perez J.M. Cell-Specific, Activatable, and Theranostic Prodrug for Dual-Targeted Cancer Imaging and Therapy, J. Am. Chem. Soc. 2011, 133, 16680-16688

Santra, S., Perez J.M. Selective N-Alkylation of Β-Alanine Facilitates the Synthesis of a Poly(amino acid)-Based Theranostic Nanoagent, Biomacromolecules 2011, 12, 3917-3927

Kaittanis, C, Santra S., Santiesteban, O.J., Henderson, T., Perez, J.M. The assembly state between magnetic nanosensors and their targets orchestrates their magnetic relaxation response, J. Am. Chem. Soc. 2011,133, 3668-3676

Asati, A., Kaittanis, C., Santra S., Perez, J.M. pH-tunable oxidase-like activity of cerium oxide nanoparticles achieving sensitive fluorigenic detection of cancer biomarkers at neutral pH, Anal. Chem. 2011, 83, 2547-2553

Kaittanis, C, Banerjee, T., Santra S., Santiesteban, O.J., Teter, K., Perez, J.M. Identification of molecular mimicry based ligands for cholera diagnostics using magnetic relaxation, Bioconj. Chem. 2011, 22, 307-314

2010

Asati, A., Santra S., Kaittanis, C, Perez, J.M. Surface-Charge-Dependent Cell Localization and Cytotoxicity of Cerium Oxide Nanoparticles, ACS Nano 2010, 4, 5321-5331

Santra, S., Kaittanis C., Perez J.M. Cytochrome c Encapsulating Theranostic Nanoparticles: A Novel Bifunctional System for targeted delivery of therapeutic membrane-impermeable proteins to tumors and imaging of cancer therapy, Mol. Pharmaceutics 2010, 7, 1209-1222.

Santra, S., Kaittanis C., Perez J.M. Aliphatic hyperbranched polyester: A new building block in the construction of multifunctional nanoparticles and nanocomposites, Langmuir 2010, 26, 5364-5373.

2009

Kaittanis C., Santra, S., Perez J.M. Role of Nanoparticle Valency in the Nondestructive Magnetic-Relaxation-Mediated Detection and Magnetic Isolation of Cells in Complex Media, J. Am. Chem. Soc. 2009, 131, 12780-12791.

Ma, L., Hong, Y., Ma, Z., Kaittanis, C., Perez, J. M. Su, M. Multiplexed highly sensitive detections of cancer biomarkers in thermal space using encapsulated phase change nanoparticles, Appl. Phys. Lett. 2009, 95, 043701.

Banerjee, S., Kar, S., Perez, J. M., Santra, S. Quantum Dot-Based OFF/ON Probe for Detection of Glutathione, J. Phys. Chem. C. 2009, 113, 9659-9663.

Santra, S., Kaittanis C., Grimm J. and Perez J.M. Drug/Dye-Loaded, Multifunctional Iron Oxide Nanoparticles for Combined Targeted Cancer Therapy and Dual Optical/MR-Imaging, Small 2009, 5, 1862-1868.

Nath, S., Kaittanis, C., Ramachandran V., Dalal. N.S., Perez, J.M. Synthesis, magnetic characterization and sensing applications of novel dextran-coated iron oxide nanorods, Chem. Mat. 2009, 21, 1761-1767.

Asati, A., Santra S., Kaittanis, C, Nath S. Perez, J.M. Oxidase-like activity of polymer-coated cerium oxide nanoparticles, Angew Chem., Int. Ed. 2009, 48, 2308-2312.

2008

Kaittanis, C., Nath, S., Perez J.M. Rapid nanoparticle-mediated monitoring of bacterial metabolic activity and assessment of antimicrobial susceptibility in blood with magnetic relaxation, PLOS One 2008, 3(9), e3253.

Perez J.M. Grimm, J, Josephson, L, Weissleder, R. Integrated nanosensors to determine levels and functional activity of human telomerase, Neoplasia 2008, 10, 1066-1072.

Perez, J.M., Asati, A., Nath, S., Kaittanis, A. Synthesis of biocompatible dextran-coated nanoceria with pH-dependent antioxidant properties, Small 2008, 4, 552-556.

Nath, S., Kaittanis, C., Tinkham, A., Perez J.M. Dextran coated gold nanoparticles for the assessment of antimicrobial susceptibility, Anal. Chem 2008, 80, 1033-1038

2007-2006

Kaittanis, C., Saleh, A. N., Perez J.M. One-step, nanoparticle-mediated Bacterial Detection with Magnetic Relaxation, NanoLetters 2007, 7, 380-383.

Rabin, O*., Perez, J.M*., Grimm, J., Wojtkiewicz, G., Weissleder, R. An X-ray computed tomography imaging agent based on long-circulating bismuth sulphide nanoparticles, Nature Mat. 2006, 5, 118-122.

2005-2000

14. McCarthy, J.R., Perez, J.M. , Bruckner C, Weissleder, R. Polymeric Nanoparticle Preparation that Erradicate Tumors, NanoLetters 2005, 5, 2552-2556.

Tsorkas, A., Newton, G., Perez, J.M., Basilion, J.P., Weissleder, R. Detection of Peroxidase/H2O2-Mediated Oxidation with Enhanced Yellow Fluorescence Protein, Anal. Chem. 2005, 77, 2862-2867.

Perez, J.M., Simeone, F.J., Tsourkas, A., Josephson, L, Weissleder, R. Peroxidase substrate nanosensors for MR imaging, NanoLetters, 2004, 4, 119-122.

Grimm, J., Perez, J.M., Josephson, L; Weissleder, R. Novel nanosensor for rapid analysis of telomerase activity, Cancer Research 2004; 64, 639-643.

Perez, J.M., Simeone, F.J., Josephson, L; Weissleder, R. Viral-induced self-assembly of magnetic nanoparticles allows the detection of viral particles in biological media, J. Amer. Chem. Soc., 2003; 125:10192-10193.

Perez, J.M., Josephson, L, O'Loughin,T., Hogemann, D., Weissleder, R. Magnetic relaxation switches capable of sensing molecular interactions, Nature Biotech., 2002; 20:816-820.

Perez, J.M., O'Loughin,T., Simeone, F.J., Weissleder, R., Josephson, L. DNA-based Magnetic Nanoparticle Assembly Acts as Magnetic relaxation Nanoswitch Allowing Screening of DNA Cleaving Agents, J. Amer. Chem. Soc., 2002; 124:2856-2857.

Josephson, L., Perez, J. M, Weissleder, R. Magnetic Nanosensors for the Detection of Oligonucleotide Sequences, Angew. Chem., Int. Ed., 2001; 40 (17), 3204-3206.

Courses

  • BCH 4054 Biochemistry II
  • MCB 4932 / IDS 5937 Foundations of Bio-imaging Science
  • BSC 3424 Nanobiotechnology

Awards, Honors, & Societies

  • NCI Career Award (K01)
  • Member, American Association for Cancer Research
  • Member, American Chemical Society
  • Member, American Association for the Advancement of Science
UCF NanoScience Technology Center | Research Pavilion 4th Floor | TEL: 407-882-1578 FAX: 407-882-2819 | 12424 Research Parkway Suite 400 Orlando, FL 32826 | nano@ucf.edu
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