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Subcellular Surveillance

Marcel Bruchez, Ph.D.
Associate Professor of Chemistry Associate Professor of Biological Sciences Carnegie Mellon University

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Light refreshments will be served


Ushaben Lal
NanoScience Technology Center
Phone: 407-882-0032
Email: usha@ucf.edu

Date: Thursday, January 30, 2014; 11:00am - 12:00pm
Cost: Free and open to the public
Location: Harris Corporation Engineering Center, Room 101A

Targeted Physiological Measurements in Cells and Organisms…
Our group has developed a chemo-genetic hybrid-tagging approach that allows us to target fluorescent dyes to specific subcellular locations in cultured cells and living zebrafish embryos. By engineering the dyes, we can produce unique ratiometric indicators that are only active when targeted to these subcellular locations and respond to changes in local pH, Ca(II) and other physiological analytes. The use of these sensors to study trafficking of GPCRs, Ion Channels and Neurotransmitter Transporters will be discussed.

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